Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.).

Evaluation of five protocols for DNA extraction from leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris.

Leaves of Malus sieversii, Vitis vinifera, and Armeniaca vulgaris contain substantial amounts of secondary metabolites, which limit the high-quality DNA extraction performance. In this study, five extraction protocols were compared for their ability to produce good quality DNA from fresh and dried (with silica gel) leaves of these species. The modified protocol of Dellaporta et al., using polyv...

Different DNA extraction methods can cause different AFLP profiles in grapevine (Vitis vinifera L.)

New species of endophytic fungi from grapevine (Vitis vinifera) in Iran

During the study on endophytic fungi of grapevine (Vitis vinifera L.), 655 fungal isolates were obtained and studied taxonomically. Based on morphological and ITS sequences data, 15 species belonging to 10 genera including Alternaria brassicicola, A. chlamydospora, A. malorum, A. atra, Arthrinium phaeospermum, A. sacchari, Aspergillus nidulans, A. wentii, Beauveria bassiana, Cheatomium elatum, ...

Detection of Polymorphism in Ancient Tempranillo Clones (Vitis vinifera L.) Using Microsatellite and Retrotransposon Markers

Tempranillo is one of the most widely cultivated grapevine varieties in Spain. After several years of clone selection, some highly recommended old clones have been identified in terms of both their qualitative and production characteristics. This study was designed to discriminate among 28 ancient clones of the cultivar Tempranillo (Vitis vinifera). DNA samples from clones were analysed using t...

Salinity Effects on Potassium Accumulation and Transporters Expression in Grape (Vitis vinifera L.). Nayer Mohammadkhani1٭, Reza Heidari2 and Nasser Abbaspour2

Hydroponically grown ten grape genotypes (Vitis vinifera L.) were treated with different concentrations of NaCl. Chawga genotype accumulated K+ in its root and shoot even at high salinity. The correlation between Na+ and K+ concentrations in root and lamina of all genotypes was negative (P